Enzyme-Protein Technology

Characterization of extracellular protease from Stenotrophomonas rhizophila MT1 isolated from aquaculture sludgewaste

A protease-producing Stenotrophomonas rhizophila MT1 isolated from sludge samples of shrimp ponds was selected to evaluate extracellular proteases. Enzyme activity reached the highest value of 139.02 U/mL after 60 h of culture. The isolate produced the highest protease activity in the culture medium containing 1% casein (w/v) with an inoculum size of 10% (v/v) and an agitation speed of 180 rpm. Zymography indicated two proteolytic bands with estimated molecular weights of 30 kDa and 110 kDa, respectively. The protease activity increased in Ca2+, Mg2+, Co2+ and K+ ions and was partially
inhibited in the presence of Mn2+, Zn2+, Cu2+ and Fe2+ ions. Meanwhile, acetone and hexane solvents
enhanced protease activity. This is the first report that evaluated the extracellular proteases produced by S. rhizophila. The isolate is a promising candidate for application in the removal of protein residues in
aquaculture sludges, minimizing the negative effect of aquaculture sludges on the environment.

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Citation: Nguyen Quang Lich, Trinh Thi Phuong Thao, Nguyen Duc Huy (2022) Characterization of extracellular protease from Stenotrophomonas rhizophila MT1 isolated from aquaculture sludgewaste. Applied Ecology and Environmental Research 20(3): 424-438