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Isolation of bacteria displaying potent antagonistic activity against fungi causes anthracnose disease in chili
Anthracnose is a common disease on many crops caused by Colletotrichum species, and it leaves serious consequences, especially on chili. Beta-1,3 glucanase is a semi-constitutive hydrolytic enzyme that is involved in the biological regulation of plant pathogens. Its main function is the breakdown of beta-1,3-glucan, a major constituent of the fungal cell wall. In a study conducted in Vietnam’s Quang Tri and Thua…
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Cloning and expression of two genes coding endo-β-1,4-glucanases from Trichoderma asperellum PQ34 in Pichia pastoris
Two genes coding endo-β-1,4-glucanases were cloned from Trichoderma asperellum PQ34 which was isolated from Thua Thien Hue province, Vietnam. The expression of these genes in Pichia pastoris produced two enzymes with molecular masses of approximately 46 kDa (about 42 kDa of enzymes and 4 kDa of signal peptide). The effects of induction time and temperature, inducer concentration, and culture medium…
