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Effects of different treatments of silver nanoparticles (AgNPs) on the growth & physiological characteristics of lotus…
Lotus (Nelumbo nucifera Gaertn.) is an aquatic perennial plant with various values, such as ornamental flowers, vegetables, food, and herbal medicine. It is cultivated and consumed throughout the different regions in Vietnam as a symbol associated with local culture. This study was conducted to evaluate the effects of four other treatments of silver nanoparticles (AgNPs) on the growth of lotus…
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Cloning and expression of two genes coding endo-β-1,4-glucanases from Trichoderma asperellum PQ34 in Pichia pastoris
Two genes coding endo-β-1,4-glucanases were cloned from Trichoderma asperellum PQ34 which was isolated from Thua Thien Hue province, Vietnam. The expression of these genes in Pichia pastoris produced two enzymes with molecular masses of approximately 46 kDa (about 42 kDa of enzymes and 4 kDa of signal peptide). The effects of induction time and temperature, inducer concentration, and culture medium…
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Development of a diagnostic scar marker for Vibrio shilonii caused acute hepatopancreatic necrosis disease in whiteleg shrimp
Background: In a previous report, we showed that Vibrio shilonii was found on whiteleg shrimp (Litopenaeus vannamei) with acute hepatopancreatic necrosis disease in Thua Thien Hue province, Vietnam. This study was performed to develop a diagnostic molecular marker generated by random amplified polymorphic DNA (RAPD) for V. shilonii rapid detection. Methods: Pathogen Vibrio spp. were isolated from shrimps and fishes, and were identified by 16S rRNA sequencing. Genetic diversity…
