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Isolation of an agricultural soil agarolytic Flavobacterium sp. AT2 and its agarase characterization
This study aimed to isolate a novel agarase-producing soil bacterium and characterize its agarose, which is known to produce biofunctional agarooligosaccharides or neo-agarooligosaccharides. A novel agar-degrading bacterium, namely AT2, was isolated from the agricultural soil and the enzyme activity was identified on a specific medium. The 16S rRNA nucleotide sequence represented 99% identity with that of the members of the…
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Isolation of bacteria displaying potent antagonistic activity against fungi causes anthracnose disease in chili
Anthracnose is a common disease on many crops caused by Colletotrichum species, and it leaves serious consequences, especially on chili. Beta-1,3 glucanase is a semi-constitutive hydrolytic enzyme that is involved in the biological regulation of plant pathogens. Its main function is the breakdown of beta-1,3-glucan, a major constituent of the fungal cell wall. In a study conducted in Vietnam’s Quang Tri and Thua…
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Characterization of extracellular protease from Stenotrophomonas rhizophila MT1 isolated from aquaculture sludgewaste
A protease-producing Stenotrophomonas rhizophila MT1 isolated from sludge samples of shrimp ponds was selected to evaluate extracellular proteases. Enzyme activity reached the highest value of 139.02 U/mL after 60 h of culture. The isolate produced the highest protease activity in the culture medium containing 1% casein (w/v) with an inoculum size of 10% (v/v) and an agitation speed of 180…
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Biodegradation and Detoxification of Malachite Green Dye by Extracellular Laccase…
The present study evaluated the potential of biodegrading the toxic synthetic dye malachite green (MG) by an enzymatic approach. Extracellular laccase derived a fungal strain Fusarium oxysporum HUIB02 was used in the direct biodegradation of MG. Time-course study showed that the crude laccase successfully removed ≥ 80% MG after 20 h of treatment. The presence of Cu2+ ions enhanced MG…