-
Genetic diversity of spotted scat (Scatophagus argus) in Vietnam based on COI genes
A spotted scat, Scatophagus argus, has a high nutritional value and is among Asia’s most widely consumed fish species. Thua Thien Hue’s consumption market considers this species to be of high economic value and requires protection and conservation of the population. However, the studies on the identification and genetic diversity of S. argus distributed in Vietnam are still lacking. Therefore, mitochondrial cytochrome c…
-
Genetic Diversity and Population Structure of the Giant Mottled Eel, Anguilla marmorata Quoy & Gaimard…
The giant mottled eel, Anguilla marmorata, is at high risk of extinction due to overfishing of glass eels and elvers to provide stock for eel farming. In Vietnam, information on the genetic diversity and population structure of this species, which is necessary for resource management, is limited. In order to address this paucity of information, sequencing of the entire mitochondrial control…
-
Cloning and expression of two genes coding endo-β-1,4-glucanases from Trichoderma asperellum PQ34 in Pichia pastoris
Two genes coding endo-β-1,4-glucanases were cloned from Trichoderma asperellum PQ34 which was isolated from Thua Thien Hue province, Vietnam. The expression of these genes in Pichia pastoris produced two enzymes with molecular masses of approximately 46 kDa (about 42 kDa of enzymes and 4 kDa of signal peptide). The effects of induction time and temperature, inducer concentration, and culture medium…
-
Development of a diagnostic scar marker for Vibrio shilonii caused acute hepatopancreatic necrosis disease in whiteleg shrimp
Background: In a previous report, we showed that Vibrio shilonii was found on whiteleg shrimp (Litopenaeus vannamei) with acute hepatopancreatic necrosis disease in Thua Thien Hue province, Vietnam. This study was performed to develop a diagnostic molecular marker generated by random amplified polymorphic DNA (RAPD) for V. shilonii rapid detection. Methods: Pathogen Vibrio spp. were isolated from shrimps and fishes, and were identified by 16S rRNA sequencing. Genetic diversity…
