Microbiology associated with disease on white lotus (Nelumbo nucifera Gaertn.) and application of silver nanoparticles for the control of plant pathogens in vitro

Many bacterial and fungal pathogens are known to affect lotus, which limits both flower quality and yield production. Treatments such as pesticides can reduce pathogens but can result in resistance. This study was conducted to identify the most common fungal, and bacterial pathogens associated with leaf spot diseases of white lotus (Nelumbo nucifera). Molecular–based identification using ITS and 16 s ribosomal DNA sequences revealed that the isolates belonged to Aspergillus aculeatus, Aspergillus fumigatus, and Klebsiella pneumoniae. We evaluated the effect of silver nanoparticles against pathogens isolated from infected white lotus leaves. According to the findings, AgNPs have antifungal activities against these plant diseases at different concentrations of 0.1,1, 10, 20, and 30 mg/L. Treatment of 30 mg/L silver nanoparticles on PDA showed the highest inhibition rate of plant pathogenic fungi Aspergillus sp., and bacteria Klebsiella pneumoniae. Based on this result, it is possible to suggest that silver nanoparticles synthesized could be an efficient, safe, cost–effective, and affordable alternative to control disease in white lotus.

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Citation: Nguyen Quang Hoang Vu, Hoang Tan Quang, Hoang Thi Kim Hong, Sakae Agarie (2022) Microbiology associated with disease on white lotus (Nelumbo nucifera Gaertn.) and application of silver nanoparticles for the control of plant pathogens in vitro. Journal of the Faculty of Agriculture, Kyushu University 67(2): 165-172.